Genotyping and primer details


Genotyping and primer details for 353F05 - At5g05250
Line ID 353F05
Locus At5g05250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1557179 of line 353F05 Chr5:1557179
   
Insertion allele amplicon 8474 - BU20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BU20: 5'-GAGAAACAGAGAGGTGGAAGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1557649
   
wt amplicon (over InsPos) 08dd - BU20
wt amplicon start Chr5:1556603
wt amplicon end Chr5:1557649
wt amplicon size 1047 bp
Locus specific primer BU20: 5'-GAGAAACAGAGAGGTGGAAGAAGA-3'
2nd locus specific primer 08dd: 5'-GATGGATCTTTTGAGGTTTTTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11