Genotyping and primer details


Genotyping and primer details for 353G10 - At3g45440
Line ID 353G10
Locus At3g45440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16665503 of line 353G10 Chr3:16665503
   
Insertion allele amplicon 8474 - 93wr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 93wr: 5'-AGCAAAGTTGTCAAAGTTTACCGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16664858
   
Insertion allele amplicon 3144 - KF43
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KF43: 5'-TTAAGCAGAAACAAGGTGTCATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16666119
   
wt amplicon (over InsPos) EQ32 - KF43
wt amplicon start Chr3:16665111
wt amplicon end Chr3:16666119
wt amplicon size 1009 bp
Locus specific primer KF43: 5'-TTAAGCAGAAACAAGGTGTCATTG-3'
2nd locus specific primer EQ32: 5'-AAAGCAACCCTAGTTTCAGTACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11