Genotyping and primer details


Genotyping and primer details for 354F03 - At3g13380
Line ID 354F03
Locus At3g13380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4347620 of line 354F03 Chr3:4347620
   
Insertion allele amplicon 8474 - EQ33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ33: 5'-TCTGTTTGCTCGTCTTGTTTCTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4347268
   
Insertion allele amplicon 3144 - KF44
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KF44: 5'-TTAACGTTTCGAGGAGCTTACAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4348008
   
Insertion allele amplicon 8474 - KF44
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KF44: 5'-TTAACGTTTCGAGGAGCTTACAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4348008
   
wt amplicon (over InsPos) KF44 - EQ33
wt amplicon start Chr3:4347268
wt amplicon end Chr3:4348008
wt amplicon size 741 bp
Locus specific primer EQ33: 5'-TCTGTTTGCTCGTCTTGTTTCTAA-3'
2nd locus specific primer KF44: 5'-TTAACGTTTCGAGGAGCTTACAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11