Genotyping and primer details


Genotyping and primer details for 356C12 - At1g21720
Line ID 356C12
Locus At1g21720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7628056 of line 356C12 Chr1:7628056
   
Insertion allele amplicon 8474 - 15ta
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15ta: 5'-AGCTTGTTAGCCATACTCCATTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7627761
   
wt amplicon (over InsPos) 20vg - 15ta
wt amplicon start Chr1:7627761
wt amplicon end Chr1:7628479
wt amplicon size 719 bp
Locus specific primer 15ta: 5'-AGCTTGTTAGCCATACTCCATTGT-3'
2nd locus specific primer 20vg: 5'-GAATGAGTCCATTACGTTACCCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11