Genotyping and primer details


Genotyping and primer details for 358B01 - At5g09350
Line ID 358B01
Locus At5g09350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2901328 of line 358B01 Chr5:2901328
   
Insertion allele amplicon 8474 - BF67
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BF67: 5'-TTGCGCTCTGTAAGTACTGGATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2901892
   
wt amplicon (over InsPos) 28rd - BF67
wt amplicon start Chr5:2901077
wt amplicon end Chr5:2901892
wt amplicon size 816 bp
Locus specific primer BF67: 5'-TTGCGCTCTGTAAGTACTGGATAA-3'
2nd locus specific primer 28rd: 5'-GGTTGTGGGATACCGAATAAAGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11