Genotyping and primer details


Genotyping and primer details for 361H10 - At4g24810
Line ID 361H10
Locus At4g24810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12786845 of line 361H10 Chr4:12786845
   
Insertion allele amplicon 8474 - 18uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18uj: 5'-GTGTTGTGAGGGATTTTTGTTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12786341
   
wt amplicon (over InsPos) 68vo - 18uj
wt amplicon start Chr4:12786341
wt amplicon end Chr4:12787112
wt amplicon size 772 bp
Locus specific primer 18uj: 5'-GTGTTGTGAGGGATTTTTGTTTTT-3'
2nd locus specific primer 68vo: 5'-ACTGCAAAAATTTGAGTGTGTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11