Genotyping and primer details


Genotyping and primer details for 362D09 - At4g32330
Line ID 362D09
Locus At4g32330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15611514 of line 362D09 Chr4:15611514
   
Insertion allele amplicon 8474 - GE22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GE22: 5'-AAAAGAGGTTAGCATTCTGGTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15610950
   
wt amplicon (over InsPos) 45wn - GE22
wt amplicon start Chr4:15610950
wt amplicon end Chr4:15612053
wt amplicon size 1104 bp
Locus specific primer GE22: 5'-AAAAGAGGTTAGCATTCTGGTTCA-3'
2nd locus specific primer 45wn: 5'-TAAAAGCACAGCAACCTTTAGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11