Genotyping and primer details


Genotyping and primer details for 363D03 - At1g67250
Line ID 363D03
Locus At1g67250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25164010 of line 363D03 Chr1:25164010
   
Insertion allele amplicon 8474 - RP01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RP01: 5'-TCTTGAATCTCTGTTGATTCATCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25164572
   
wt amplicon (over InsPos) 02lw - RP01
wt amplicon start Chr1:25163752
wt amplicon end Chr1:25164572
wt amplicon size 821 bp
Locus specific primer RP01: 5'-TCTTGAATCTCTGTTGATTCATCG-3'
2nd locus specific primer 02lw: 5'-ATACACGATGAGTCATGATCCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11