Genotyping and primer details


Genotyping and primer details for 365C11 - At1g04580
Line ID 365C11
Locus At1g04580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1255116 of line 365C11 Chr1:1255116
   
Insertion allele amplicon 8474 - CD84
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD84: 5'-ATTGGAAGTGCATTGCCTTATATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1255742
   
Insertion allele amplicon 8474 - CD85
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD85: 5'-GTGTCTGCAACCTGATAGGTCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1254740
   
wt amplicon (over InsPos) CD85 - CD84
wt amplicon start Chr1:1254740
wt amplicon end Chr1:1255742
wt amplicon size 1003 bp
Locus specific primer CD84: 5'-ATTGGAAGTGCATTGCCTTATATC-3'
2nd locus specific primer CD85: 5'-GTGTCTGCAACCTGATAGGTCATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11