Genotyping and primer details


Genotyping and primer details for 366B01 - At1g44780
Line ID 366B01
Locus At1g44780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:16911874 of line 366B01 Chr1:16911874
   
Insertion allele amplicon 8474 - BW58
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BW58: 5'-ACTGCAACTGATGTGTATGGAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:16911222
   
Insertion allele amplicon 8474 - BW59
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BW59: 5'-ATCACATCATTGAGTGGGTATCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:16912281
   
wt amplicon (over InsPos) BW59 - BW58
wt amplicon start Chr1:16911222
wt amplicon end Chr1:16912281
wt amplicon size 1060 bp
Locus specific primer BW58: 5'-ACTGCAACTGATGTGTATGGAAAG-3'
2nd locus specific primer BW59: 5'-ATCACATCATTGAGTGGGTATCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11