Genotyping and primer details


Genotyping and primer details for 367A04 - At1g64110
Line ID 367A04
Locus At1g64110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23799442 of line 367A04 Chr1:23799442
   
Insertion allele amplicon 8474 - KY01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KY01: 5'-TTTCCGTTTCTGTACTCAGGATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23798804
   
wt amplicon (over InsPos) 15gl - KY01
wt amplicon start Chr1:23798804
wt amplicon end Chr1:23799939
wt amplicon size 1136 bp
Locus specific primer KY01: 5'-TTTCCGTTTCTGTACTCAGGATCT-3'
2nd locus specific primer 15gl: 5'-TCCTCAGAGAGAAGAGTCAAAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11