Genotyping and primer details


Genotyping and primer details for 368C08 - At1g71410
Line ID 368C08
Locus At1g71410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26913343 of line 368C08 Chr1:26913343
   
Insertion allele amplicon 8474 - 15dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15dr: 5'-GCCAACTTACCAACAGAAAATGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26912718
   
wt amplicon (over InsPos) 16ej - 15dr
wt amplicon start Chr1:26912718
wt amplicon end Chr1:26913772
wt amplicon size 1055 bp
Locus specific primer 15dr: 5'-GCCAACTTACCAACAGAAAATGAT-3'
2nd locus specific primer 16ej: 5'-AAATCCTGCTTCTTCTCGTCATAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11