Genotyping and primer details


Genotyping and primer details for 368D02 - At2g29900
Line ID 368D02
Locus At2g29900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12750182 of line 368D02 Chr2:12750182
   
Insertion allele amplicon 8474 - 20et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20et: 5'-TCTCTCCAAACTCTTCTCTGAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12750579
   
wt amplicon (over InsPos) 02fz - 20et
wt amplicon start Chr2:12749687
wt amplicon end Chr2:12750579
wt amplicon size 893 bp
Locus specific primer 20et: 5'-TCTCTCCAAACTCTTCTCTGAACC-3'
2nd locus specific primer 02fz: 5'-TTTGGGGAATGACTTGAATAAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11