Genotyping and primer details


Genotyping and primer details for 372C05 - At2g37010
Line ID 372C05
Locus At2g37010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15542900 of line 372C05 Chr2:15542900
   
Insertion allele amplicon 8474 - 12du
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12du: 5'-TCTGATTTATGTAAACGAAGCGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15542545
   
wt amplicon (over InsPos) 06es - 12du
wt amplicon start Chr2:15542545
wt amplicon end Chr2:15543327
wt amplicon size 783 bp
Locus specific primer 12du: 5'-TCTGATTTATGTAAACGAAGCGTG-3'
2nd locus specific primer 06es: 5'-CTTCTCTCGACTTTGCTTGTCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11