Genotyping and primer details


Genotyping and primer details for 373G03 - At3g01520
Line ID 373G03
Locus At3g01520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:209901 of line 373G03 Chr3:209901
   
Insertion allele amplicon 8474 - 38ns
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 38ns: 5'-TAGCTGTGCAAAACTTGTAGGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:209340
   
wt amplicon (over InsPos) 55vh - 38ns
wt amplicon start Chr3:209340
wt amplicon end Chr3:210201
wt amplicon size 862 bp
Locus specific primer 38ns: 5'-TAGCTGTGCAAAACTTGTAGGTTG-3'
2nd locus specific primer 55vh: 5'-GGTGGTGTTTGAGTAACTTCTGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11