Genotyping and primer details


Genotyping and primer details for 375B05 - At4g32342
Line ID 375B05
Locus At4g32342 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15615472 of line 375B05 Chr4:15615472
   
Insertion allele amplicon 8474 - 11wu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11wu: 5'-AAGTACGTGTGATATGGACCACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15615931
   
wt amplicon (over InsPos) 26xd - 11wu
wt amplicon start Chr4:15614837
wt amplicon end Chr4:15615931
wt amplicon size 1095 bp
Locus specific primer 11wu: 5'-AAGTACGTGTGATATGGACCACAA-3'
2nd locus specific primer 26xd: 5'-ATTGATGAGAAATGACTGACAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11