Genotyping and primer details


Genotyping and primer details for 379D03 - At1g72500
Line ID 379D03
Locus At1g72500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27296867 of line 379D03 Chr1:27296867
   
Insertion allele amplicon 8474 - 39by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 39by: 5'-AAGTTACCTCAACTGATCGGAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27296256
   
wt amplicon (over InsPos) 03dq - 39by
wt amplicon start Chr1:27296256
wt amplicon end Chr1:27297134
wt amplicon size 879 bp
Locus specific primer 39by: 5'-AAGTTACCTCAACTGATCGGAGAA-3'
2nd locus specific primer 03dq: 5'-AACGAAGAGTTGTGTTTGTGATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11