Genotyping and primer details


Genotyping and primer details for 381D03 - At3g05590
Line ID 381D03
Locus At3g05590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1622823 of line 381D03 Chr3:1622823
   
Insertion allele amplicon 8474 - 15lu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15lu: 5'-CGTTGAAACCCTCTCTCTCTACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1622132
   
wt amplicon (over InsPos) 03mo - 15lu
wt amplicon start Chr3:1622132
wt amplicon end Chr3:1623341
wt amplicon size 1210 bp
Locus specific primer 15lu: 5'-CGTTGAAACCCTCTCTCTCTACTG-3'
2nd locus specific primer 03mo: 5'-GCTGGATTGAAGATTTCAAAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11