Genotyping and primer details


Genotyping and primer details for 381E02 - At1g29970
Line ID 381E02
Locus At1g29970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10500077 of line 381E02 Chr1:10500077
   
Insertion allele amplicon 8474 - AG06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AG06: 5'-TTAAGTGACAGTTCGTTGTGGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10500619
   
Insertion allele amplicon 8474 - AG07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AG07: 5'-GAACTTTTTGTGTAGCAATGGTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10499692
   
wt amplicon (over InsPos) AG07 - AG06
wt amplicon start Chr1:10499692
wt amplicon end Chr1:10500619
wt amplicon size 928 bp
Locus specific primer AG06: 5'-TTAAGTGACAGTTCGTTGTGGAAC-3'
2nd locus specific primer AG07: 5'-GAACTTTTTGTGTAGCAATGGTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11