Genotyping and primer details


Genotyping and primer details for 382E10 - At1g73680
Line ID 382E10
Locus At1g73680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27706751 of line 382E10 Chr1:27706751
   
Insertion allele amplicon 8474 - 26vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26vf: 5'-TTTCCAGTGTTATTAGGAGTGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27707109
   
wt amplicon (over InsPos) 43vw - 26vf
wt amplicon start Chr1:27706013
wt amplicon end Chr1:27707109
wt amplicon size 1097 bp
Locus specific primer 26vf: 5'-TTTCCAGTGTTATTAGGAGTGGCT-3'
2nd locus specific primer 43vw: 5'-GAACTTGAATGACTTCAATGGACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11