Genotyping and primer details

Genotyping and primer details for 382G07 - At1g17590
Line ID 382G07
Locus At1g17590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6052422 of line 382G07 Chr1:6052422
Insertion allele amplicon 8474 - 44vk
Locus specific primer 44vk: 5'-ACTCAGTGATTCAGTCACACCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6051868
wt amplicon (over InsPos) 67vo - 44vk
wt amplicon start Chr1:6051868
wt amplicon end Chr1:6052626
wt amplicon size 759 bp
Locus specific primer 44vk: 5'-ACTCAGTGATTCAGTCACACCATT-3'
2nd locus specific primer 67vo: 5'-GGTACTGCTAATAATGGAGCTGGT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11