Genotyping and primer details


Genotyping and primer details for 383H03 - At1g12730
Line ID 383H03
Locus At1g12730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4337343 of line 383H03 Chr1:4337343
   
Insertion allele amplicon 8474 - 14ad
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14ad: 5'-ACCATCTTTAATGTTCCGGTTAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4336803
   
wt amplicon (over InsPos) 01tl - 14ad
wt amplicon start Chr1:4336803
wt amplicon end Chr1:4337735
wt amplicon size 933 bp
Locus specific primer 14ad: 5'-ACCATCTTTAATGTTCCGGTTAAA-3'
2nd locus specific primer 01tl: 5'-CTCCTAATACATGTTGTTCAACGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11