Genotyping and primer details


Genotyping and primer details for 384G06 - At3g44990
Line ID 384G06
Locus At3g44990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16448336 of line 384G06 Chr3:16448336
   
Insertion allele amplicon 8474 - 27fb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27fb: 5'-CTAGCTCTCTTAGTGTTGTGCCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16448657
   
wt amplicon (over InsPos) 34gc - 27fb
wt amplicon start Chr3:16447770
wt amplicon end Chr3:16448657
wt amplicon size 888 bp
Locus specific primer 27fb: 5'-CTAGCTCTCTTAGTGTTGTGCCCT-3'
2nd locus specific primer 34gc: 5'-GAGTAGGGTCAAACCACAAGGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11