Genotyping and primer details


Genotyping and primer details for 386G06 - At1g29060
Line ID 386G06
Locus At1g29060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10148020 of line 386G06 Chr1:10148020
   
Insertion allele amplicon 8474 - 09jr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09jr: 5'-CTTGAGCAATCTGCATCAAAATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10148612
   
wt amplicon (over InsPos) 19kj - 09jr
wt amplicon start Chr1:10147405
wt amplicon end Chr1:10148612
wt amplicon size 1208 bp
Locus specific primer 09jr: 5'-CTTGAGCAATCTGCATCAAAATAA-3'
2nd locus specific primer 19kj: 5'-TGGGTATCGTTGATGCTTACAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11