Genotyping and primer details


Genotyping and primer details for 387B07 - At2g41830
Line ID 387B07
Locus At2g41830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17452618 of line 387B07 Chr2:17452618
   
Insertion allele amplicon 8474 - 11wm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11wm: 5'-CATCAATGAAATATCTCGCAAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17452202
   
wt amplicon (over InsPos) 25xd - 11wm
wt amplicon start Chr2:17452202
wt amplicon end Chr2:17453276
wt amplicon size 1075 bp
Locus specific primer 11wm: 5'-CATCAATGAAATATCTCGCAAAGA-3'
2nd locus specific primer 25xd: 5'-GTTACTTCAAGCTATGGTCCATCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11