Genotyping and primer details


Genotyping and primer details for 390A04 - At2g44650
Line ID 390A04
Locus At2g44650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18420592 of line 390A04 Chr2:18420592
   
Insertion allele amplicon 8474 - 37jd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 37jd: 5'-ATCAAGAGCAAGTTTAGGATTGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18421032
   
wt amplicon (over InsPos) 08jz - 37jd
wt amplicon start Chr2:18420146
wt amplicon end Chr2:18421032
wt amplicon size 887 bp
Locus specific primer 37jd: 5'-ATCAAGAGCAAGTTTAGGATTGGA-3'
2nd locus specific primer 08jz: 5'-CAAGACGAACAAGAACTCTGTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11