Genotyping and primer details


Genotyping and primer details for 392F12 - At1g11930
Line ID 392F12
Locus At1g11930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4028805 of line 392F12 Chr1:4028805
   
Insertion allele amplicon 8474 - 46ns
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 46ns: 5'-GAAGCTGCAAATTTCAATGTATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4029145
   
wt amplicon (over InsPos) 28oj - 46ns
wt amplicon start Chr1:4028332
wt amplicon end Chr1:4029145
wt amplicon size 814 bp
Locus specific primer 46ns: 5'-GAAGCTGCAAATTTCAATGTATCA-3'
2nd locus specific primer 28oj: 5'-TTTGATGGTTTATAGACTTTGCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11