Genotyping and primer details


Genotyping and primer details for 400C02 - At1g62130
Line ID 400C02
Locus At1g62130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22962501 of line 400C02 Chr1:22962501
   
Insertion allele amplicon 8474 - 18dh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18dh: 5'-ACGGTTCTTGATTTCTTCAAGTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22962008
   
wt amplicon (over InsPos) 50dy - 18dh
wt amplicon start Chr1:22962008
wt amplicon end Chr1:22962977
wt amplicon size 970 bp
Locus specific primer 18dh: 5'-ACGGTTCTTGATTTCTTCAAGTGT-3'
2nd locus specific primer 50dy: 5'-TGAGGTTGCTAGTATGACAAATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11