Genotyping and primer details


Genotyping and primer details for 402B12 - At3g60180
Line ID 402B12
Locus At3g60180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22243967 of line 402B12 Chr3:22243967
   
Insertion allele amplicon 8474 - 18qp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18qp: 5'-TTGCTTGGGTAAGATGATAAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22244383
   
wt amplicon (over InsPos) 43sq - 18qp
wt amplicon start Chr3:22243618
wt amplicon end Chr3:22244383
wt amplicon size 766 bp
Locus specific primer 18qp: 5'-TTGCTTGGGTAAGATGATAAAACC-3'
2nd locus specific primer 43sq: 5'-CTCACTGAAGCATGTACTTACCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11