Genotyping and primer details


Genotyping and primer details for 408A01 - At4g28890
Line ID 408A01
Locus At4g28890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14256801 of line 408A01 Chr4:14256801
   
Insertion allele amplicon 8474 - 38uw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 38uw: 5'-GGAGCTTTACATCGAGAGAGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14257145
   
wt amplicon (over InsPos) 65vl - 38uw
wt amplicon start Chr4:14256620
wt amplicon end Chr4:14257145
wt amplicon size 526 bp
Locus specific primer 38uw: 5'-GGAGCTTTACATCGAGAGAGAAGA-3'
2nd locus specific primer 65vl: 5'-CGATATACTGAGTCTAGGAACCGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11