Genotyping and primer details


Genotyping and primer details for 409E07 - At1g64610
Line ID 409E07
Locus At1g64610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24000530 of line 409E07 Chr1:24000530
   
Insertion allele amplicon 8474 - CB69
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CB69: 5'-AAGGAAGAACAAAAGTACAGGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24000076
   
wt amplicon (over InsPos) 02pc - CB69
wt amplicon start Chr1:24000076
wt amplicon end Chr1:24001054
wt amplicon size 979 bp
Locus specific primer CB69: 5'-AAGGAAGAACAAAAGTACAGGCAA-3'
2nd locus specific primer 02pc: 5'-TCTGGTGTGAAAGAGATTGATTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11