Genotyping and primer details


Genotyping and primer details for 411H07 - At2g34370
Line ID 411H07
Locus At2g34370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14510614 of line 411H07 Chr2:14510614
   
Insertion allele amplicon 8474 - BJ52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BJ52: 5'-CAACAAAGATTCATCAGTGTTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14511344
   
wt amplicon (over InsPos) 01rt - BJ52
wt amplicon start Chr2:14510277
wt amplicon end Chr2:14511344
wt amplicon size 1068 bp
Locus specific primer BJ52: 5'-CAACAAAGATTCATCAGTGTTTCC-3'
2nd locus specific primer 01rt: 5'-CTTTAAAAACGGTTGCCACTTATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11