Genotyping and primer details


Genotyping and primer details for 413B02 - At4g38360
Line ID 413B02
Locus At4g38360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17967745 of line 413B02 Chr4:17967745
   
Insertion allele amplicon 8474 - 12gz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12gz: 5'-AGAACTACAAGATGCGGAACCTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17968104
   
wt amplicon (over InsPos) 12hv - 12gz
wt amplicon start Chr4:17967232
wt amplicon end Chr4:17968104
wt amplicon size 873 bp
Locus specific primer 12gz: 5'-AGAACTACAAGATGCGGAACCTAA-3'
2nd locus specific primer 12hv: 5'-ACCAAACTTCATGTCATTAGGTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11