Genotyping and primer details


Genotyping and primer details for 415B04 - At3g49220
Line ID 415B04
Locus At3g49220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18251245 of line 415B04 Chr3:18251245
   
Insertion allele amplicon 8474 - 16nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16nt: 5'-TTTTTAAAACCCTATCACTCCGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18251566
   
wt amplicon (over InsPos) 06or - 16nt
wt amplicon start Chr3:18250993
wt amplicon end Chr3:18251566
wt amplicon size 574 bp
Locus specific primer 16nt: 5'-TTTTTAAAACCCTATCACTCCGAA-3'
2nd locus specific primer 06or: 5'-TTAATTTGATGTTCGTTGGAGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11