Genotyping and primer details


Genotyping and primer details for 415G01 - At2g21380
Line ID 415G01
Locus At2g21380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9148264 of line 415G01 Chr2:9148264
   
Insertion allele amplicon 8474 - 18dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18dr: 5'-AGCTGAAGAAGCAAAGAGAAGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9147816
   
wt amplicon (over InsPos) 13ej - 18dr
wt amplicon start Chr2:9147816
wt amplicon end Chr2:9148587
wt amplicon size 772 bp
Locus specific primer 18dr: 5'-AGCTGAAGAAGCAAAGAGAAGAGA-3'
2nd locus specific primer 13ej: 5'-ATCTCTTGCATTCTTGCCTGTTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11