Genotyping and primer details


Genotyping and primer details for 415H01 - At3g01200
Line ID 415H01
Locus At3g01200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:70558 of line 415H01 Chr3:70558
   
Insertion allele amplicon 8474 - 38sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 38sj: 5'-TTTGAGTAGCAAATGAGAATGGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:70032
   
wt amplicon (over InsPos) 04sz - 38sj
wt amplicon start Chr3:70032
wt amplicon end Chr3:70974
wt amplicon size 943 bp
Locus specific primer 38sj: 5'-TTTGAGTAGCAAATGAGAATGGTG-3'
2nd locus specific primer 04sz: 5'-TCTCTTCTCCTGGGTAAATGAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11