Genotyping and primer details


Genotyping and primer details for 420H08 - At1g63300
Line ID 420H08
Locus At1g63300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23483274 of line 420H08 Chr1:23483274
   
Insertion allele amplicon 8474 - 31lm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31lm: 5'-TCGTTCTTAAGCTTCTCTACCTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23483669
   
wt amplicon (over InsPos) 12mj - 31lm
wt amplicon start Chr1:23482817
wt amplicon end Chr1:23483669
wt amplicon size 853 bp
Locus specific primer 31lm: 5'-TCGTTCTTAAGCTTCTCTACCTCG-3'
2nd locus specific primer 12mj: 5'-TGCAATGTTTCTCTTCCATTACAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11