Genotyping and primer details


Genotyping and primer details for 422C11 - At3g43110
Line ID 422C11
Locus At3g43110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:15091833 of line 422C11 Chr3:15091833
   
Insertion allele amplicon 8474 - JG63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JG63: 5'-TACGTACCCAAACGTACATACCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:15091260
   
wt amplicon (over InsPos) AE04 - JG63
wt amplicon start Chr3:15091260
wt amplicon end Chr3:15092335
wt amplicon size 1076 bp
Locus specific primer JG63: 5'-TACGTACCCAAACGTACATACCAA-3'
2nd locus specific primer AE04: 5'-AACCACTGATTCAATCTTCTTCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11