Genotyping and primer details


Genotyping and primer details for 422D09 - At5g11880
Line ID 422D09
Locus At5g11880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3829919 of line 422D09 Chr5:3829919
   
Insertion allele amplicon 8474 - KF13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KF13: 5'-CCAATAACAGATCTCACTCCTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3829566
   
Insertion allele amplicon 8474 - KF14
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KF14: 5'-CTTCATCGTTAGCATTCAACAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3830367
   
wt amplicon (over InsPos) KF14 - KF13
wt amplicon start Chr5:3829566
wt amplicon end Chr5:3830367
wt amplicon size 801 bp
Locus specific primer KF13: 5'-CCAATAACAGATCTCACTCCTTCC-3'
2nd locus specific primer KF14: 5'-CTTCATCGTTAGCATTCAACAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11