Genotyping and primer details


Genotyping and primer details for 423A05 - At3g56590
Line ID 423A05
Locus At3g56590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20966585 of line 423A05 Chr3:20966585
   
Insertion allele amplicon 8474 - 13gd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13gd: 5'-AGTTGGTGCGTAGTTTAGTGTGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20965987
   
wt amplicon (over InsPos) 24sl - 13gd
wt amplicon start Chr3:20965987
wt amplicon end Chr3:20966919
wt amplicon size 933 bp
Locus specific primer 13gd: 5'-AGTTGGTGCGTAGTTTAGTGTGTG-3'
2nd locus specific primer 24sl: 5'-TCTTGCGAATACAAGTGTCATACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11