Genotyping and primer details


Genotyping and primer details for 425C03 - At2g42330
Line ID 425C03
Locus At2g42330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17632391 of line 425C03 Chr2:17632391
   
Insertion allele amplicon 8474 - PB36
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PB36: 5'-TGCTTCTTTAAAACTCAGCTCCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17632044
   
wt amplicon (over InsPos) 25da - PB36
wt amplicon start Chr2:17632044
wt amplicon end Chr2:17632811
wt amplicon size 768 bp
Locus specific primer PB36: 5'-TGCTTCTTTAAAACTCAGCTCCTC-3'
2nd locus specific primer 25da: 5'-AGATTGCTAGAGACATGGGAGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11