Genotyping and primer details


Genotyping and primer details for 425D02 - At3g48780
Line ID 425D02
Locus At3g48780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18092156 of line 425D02 Chr3:18092156
   
Insertion allele amplicon 8474 - BC22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BC22: 5'-ACCTCCTATGTTCAACGGATAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18091835
   
wt amplicon (over InsPos) 55rd - BC22
wt amplicon start Chr3:18091835
wt amplicon end Chr3:18092732
wt amplicon size 898 bp
Locus specific primer BC22: 5'-ACCTCCTATGTTCAACGGATAATG-3'
2nd locus specific primer 55rd: 5'-ACGAGTGCATCAGTATTCACAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11