Genotyping and primer details


Genotyping and primer details for 426D03 - At5g47070
Line ID 426D03
Locus At5g47070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19119513 of line 426D03 Chr5:19119513
   
Insertion allele amplicon 8474 - 14jr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14jr: 5'-CTGCGGTAGTGACATGAGTATTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19119259
   
wt amplicon (over InsPos) 18kj - 14jr
wt amplicon start Chr5:19119259
wt amplicon end Chr5:19120201
wt amplicon size 943 bp
Locus specific primer 14jr: 5'-CTGCGGTAGTGACATGAGTATTGT-3'
2nd locus specific primer 18kj: 5'-GATTCTTCTGATCCTCCACTTGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11