Genotyping and primer details


Genotyping and primer details for 431A04 - At2g18360
Line ID 431A04
Locus At2g18360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7977194 of line 431A04 Chr2:7977194
   
Insertion allele amplicon 8474 - BC18
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BC18: 5'-AGTGGATTCAATATGTCTTCGGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7976595
   
wt amplicon (over InsPos) 02se - BC18
wt amplicon start Chr2:7976595
wt amplicon end Chr2:7977593
wt amplicon size 999 bp
Locus specific primer BC18: 5'-AGTGGATTCAATATGTCTTCGGAC-3'
2nd locus specific primer 02se: 5'-TTCAAGCAAAATTACAGGTGTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11