Genotyping and primer details


Genotyping and primer details for 431G10 - At4g00900
Line ID 431G10
Locus At4g00900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:383039 of line 431G10 Chr4:383039
   
Insertion allele amplicon 8474 - 32bk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32bk: 5'-ACTGTGTCTTTGGTTGAGGTTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:382603
   
wt amplicon (over InsPos) 12cr - 32bk
wt amplicon start Chr4:382603
wt amplicon end Chr4:383410
wt amplicon size 808 bp
Locus specific primer 32bk: 5'-ACTGTGTCTTTGGTTGAGGTTTCT-3'
2nd locus specific primer 12cr: 5'-TCAGCTTCTTTGGGTTAATCTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11