Genotyping and primer details


Genotyping and primer details for 431H06 - At5g03300
Line ID 431H06
Locus At5g03300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:796591 of line 431H06 Chr5:796591
   
Insertion allele amplicon 8474 - 39sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 39sj: 5'-TTTAACCCATCAAATACCAAAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:796955
   
wt amplicon (over InsPos) 03sz - 39sj
wt amplicon start Chr5:796358
wt amplicon end Chr5:796955
wt amplicon size 598 bp
Locus specific primer 39sj: 5'-TTTAACCCATCAAATACCAAAAGC-3'
2nd locus specific primer 03sz: 5'-ATTAGTCGGTCCACAAAGAAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11