Genotyping and primer details


Genotyping and primer details for 436B01 - At1g26665
Line ID 436B01
Locus At1g26665 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9214585 of line 436B01 Chr1:9214585
   
Insertion allele amplicon 8474 - CC22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CC22: 5'-AAAGGAGTCACCTTAAAAGCATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9215104
   
wt amplicon (over InsPos) 31si - CC22
wt amplicon start Chr1:9214246
wt amplicon end Chr1:9215104
wt amplicon size 859 bp
Locus specific primer CC22: 5'-AAAGGAGTCACCTTAAAAGCATCA-3'
2nd locus specific primer 31si: 5'-CAAACGCATCTCTGTTGTAGGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11