Genotyping and primer details


Genotyping and primer details for 438B09 - At3g50700
Line ID 438B09
Locus At3g50700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18841731 of line 438B09 Chr3:18841731
   
Insertion allele amplicon 8474 - 28aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28aq: 5'-CCTGGTAAACTTCAAAAATCCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18841071
   
wt amplicon (over InsPos) 08nv - 28aq
wt amplicon start Chr3:18841071
wt amplicon end Chr3:18842321
wt amplicon size 1251 bp
Locus specific primer 28aq: 5'-CCTGGTAAACTTCAAAAATCCAAA-3'
2nd locus specific primer 08nv: 5'-TATATACTTGGAGAAGCCGAGGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11