Genotyping and primer details


Genotyping and primer details for 443B12 - At5g05990
Line ID 443B12
Locus At5g05990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1807423 of line 443B12 Chr5:1807423
   
Insertion allele amplicon 8474 - 12mh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12mh: 5'-GGGAAATAAAAACCCTCACTCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1807944
   
wt amplicon (over InsPos) 10mx - 12mh
wt amplicon start Chr5:1806975
wt amplicon end Chr5:1807944
wt amplicon size 970 bp
Locus specific primer 12mh: 5'-GGGAAATAAAAACCCTCACTCTTC-3'
2nd locus specific primer 10mx: 5'-ATCATGTACTCGTGCAAGAAGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11