Genotyping and primer details


Genotyping and primer details for 443D10 - At2g33500
Line ID 443D10
Locus At2g33500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14190225 of line 443D10 Chr2:14190225
   
Insertion allele amplicon 8474 - 03pt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03pt: 5'-GAATTCATGGCTGGATTTAGGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14190588
   
wt amplicon (over InsPos) 02qh - 03pt
wt amplicon start Chr2:14189812
wt amplicon end Chr2:14190588
wt amplicon size 777 bp
Locus specific primer 03pt: 5'-GAATTCATGGCTGGATTTAGGATA-3'
2nd locus specific primer 02qh: 5'-CACACTCCTGACACAATACGAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11